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Alkaline Copper Solution
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What is the primary effect of using an alkaline copper solution in protein analysis?

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A

It increases the solubility of proteins in water

B

It facilitates the formation of colored complexes with proteins

C

It denatures proteins, making them easier to measure

D

It reduces the absorption of light by proteins in spectrophotometry

Understanding the Answer

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The copper ions in the alkaline solution bind to protein molecules and create a colored complex. Other options are incorrect because Students often think the solution makes proteins dissolve better; The main goal is not to break the protein structure.

Key Concepts

Protein analysis
Colorimetric detection
Spectrophotometry
Topic

Alkaline Copper Solution

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medium level question

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Definition
Definition

Alkaline copper solution plays a critical role in protein analysis methods, facilitating the colorimetric detection of proteins in biological samples. This solution interacts with protein samples to form complexes that can be quantified using spectrophotometry, contributing to the accurate measurement of protein concentrations.

Topic Definition

Alkaline copper solution plays a critical role in protein analysis methods, facilitating the colorimetric detection of proteins in biological samples. This solution interacts with protein samples to form complexes that can be quantified using spectrophotometry, contributing to the accurate measurement of protein concentrations.

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