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Question & Answer
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It sequences only specific genes of interest
It provides a comprehensive view of all genetic material in a sample
It requires a larger amount of DNA than other methods
It can only identify known species in a sample
Understanding the Answer
Let's break down why this is correct
Whole‑genome shotgun sequencing reads every piece of DNA in a sample, not just a few genes. Other options are incorrect because Many think shotgun targets only specific genes, but it actually samples random fragments from the whole genome; Some believe shotgun needs more DNA, but the amount needed is similar to other sequencing methods.
Key Concepts
Whole-Genome Shotgun Metagenomic Sequencing
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Deep Dive: Whole-Genome Shotgun Metagenomic Sequencing
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Definition
Whole-genome shotgun metagenomic sequencing is a technique that involves sequencing all DNA present in a microbial sample. This approach provides a comprehensive view of the genetic content of a microbial community, allowing for the identification of species and functional analysis.
Topic Definition
Whole-genome shotgun metagenomic sequencing is a technique that involves sequencing all DNA present in a microbial sample. This approach provides a comprehensive view of the genetic content of a microbial community, allowing for the identification of species and functional analysis.
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