Learning Path
Question & Answer
Choose the Best Answer
The presence of interfering substances that absorb at the same wavelength
The protein sample was too dilute to detect
The assay was performed at the wrong temperature
The reagents used were expired and ineffective
Understanding the Answer
Let's break down why this is correct
When other chemicals in the sample absorb light at the same wavelength as the protein dye, the measured color is weaker than it should be. Other options are incorrect because A sample that is too dilute still gives a measurable color if the assay is sensitive enough; Temperature can affect the reaction speed, but most colorimetric kits are designed to work at room temperature or a set range.
Key Concepts
Protein Measurement Methods
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Deep Dive: Protein Measurement Methods
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Definition
Protein measurement methods involve various techniques and reagents to quantify protein content in biological samples. This includes procedures for measuring proteins in solution, after precipitation, and determining minimal protein concentrations. Understanding the principles and limitations of protein measurement methods is crucial for accurate analysis.
Topic Definition
Protein measurement methods involve various techniques and reagents to quantify protein content in biological samples. This includes procedures for measuring proteins in solution, after precipitation, and determining minimal protein concentrations. Understanding the principles and limitations of protein measurement methods is crucial for accurate analysis.
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