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Alkaline Copper Solution
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How does the alkaline copper solution contribute to the colorimetric detection of proteins in biological samples?

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A

It reacts with amino acids to produce a light-absorbing complex.

B

It denatures proteins, making them easier to measure.

C

It forms complexes with proteins that can be quantified spectrophotometrically.

D

It neutralizes acids in the sample to stabilize protein structures.

Understanding the Answer

Let's break down why this is correct

The solution contains copper ions in a basic (alkaline) environment. Other options are incorrect because The misconception is that the solution reacts only with individual amino acids; The idea that it denatures proteins to make them easier to measure is incorrect.

Key Concepts

Protein Analysis
Spectrophotometry
Colorimetric Detection
Topic

Alkaline Copper Solution

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hard level question

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Definition
Definition

Alkaline copper solution plays a critical role in protein analysis methods, facilitating the colorimetric detection of proteins in biological samples. This solution interacts with protein samples to form complexes that can be quantified using spectrophotometry, contributing to the accurate measurement of protein concentrations.

Topic Definition

Alkaline copper solution plays a critical role in protein analysis methods, facilitating the colorimetric detection of proteins in biological samples. This solution interacts with protein samples to form complexes that can be quantified using spectrophotometry, contributing to the accurate measurement of protein concentrations.

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