Which of the following factors most significantly contributed to the assimilation challenges faced by the Lost Colony of Roanoke during the English colonization efforts?

The Lost Colony of Roanoke faced significant assimilation challenges mainly because of a lack of resources and support from England. When the first group of settlers arrived, they were unprepared for the harsh conditions and did not have enough food or tools. Additionally, they struggled to communicate effectively with the local Native American tribes, which made it hard to establish friendly relationships. For example, when some settlers tried to trade with the tribes, misunderstandings often led to conflicts instead of cooperation. As a result, these challenges made it difficult for the colony to survive and adapt to its new environment.