What was one of the major factors contributing to the difficulties faced by the Lost Colony of Roanoke?

One major factor that contributed to the difficulties faced by the Lost Colony of Roanoke was the lack of adequate supplies and support from England. When the colonists arrived, they were not fully prepared for the harsh conditions of the New World, including food shortages and limited resources. For example, the first group of settlers struggled to grow crops because they were unfamiliar with the land and its climate. Additionally, when their leader, John White, returned to England for more supplies, he was delayed for three years due to conflicts in Europe, leaving the colony without help. This combination of poor planning and lack of timely support ultimately led to the colony's mysterious disappearance.