John White faced numerous challenges while attempting to establish a colony at Roanoke, including conflicts with __________, which significantly impacted the colony's survival.

John White faced numerous challenges while trying to establish a colony at Roanoke, including conflicts with local Native American tribes. These conflicts made it difficult for the colonists to find food and resources, which were essential for their survival. For example, when the settlers tried to trade with the Native Americans, misunderstandings and tensions sometimes led to violence, making cooperation nearly impossible. Without a stable relationship with the indigenous people, the colonists struggled to thrive in their new environment. This struggle ultimately contributed to the mysterious disappearance of the Roanoke colony, as they could not secure the support they needed to survive.