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CRISPR-Cas9 Genome Editing
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If CRISPR-Cas9 successfully edits a gene to confer antibiotic resistance in bacteria, what is the most likely underlying mechanism that enables this change?

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A

The Cas9 protein randomly alters DNA sequences throughout the genome.

B

The guide RNA directs the Cas9 to a specific DNA sequence, enabling precise cuts.

C

The PAM site is a non-specific region that allows multiple edits at once.

D

The bacteria naturally develop resistance without any genetic modifications.

Understanding the Answer

Let's break down why this is correct

The guide RNA is made to match a specific DNA sequence. Other options are incorrect because Some think Cas9 changes DNA everywhere; The PAM site is a short DNA motif that Cas9 needs to bind.

Key Concepts

CRISPR-Cas9 Mechanism
Gene Editing Applications
Antibiotic Resistance
Topic

CRISPR-Cas9 Genome Editing

Difficulty

hard level question

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understand

Deep Dive: CRISPR-Cas9 Genome Editing

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Definition
Definition

CRISPR-Cas9 Genome Editing is a revolutionary method used in genetic engineering that enables precise modifications of DNA sequences in living organisms. This technology utilizes the Cas9 protein, which acts as molecular scissors to cut DNA at specific sites, guided by a sequence known as Protospacer or guide RNA. The significance of this method in business lies in its potential applications across various fields, including medicine, agriculture, and biotechnology, allowing companies to innovate and develop solutions for complex challenges.

Topic Definition

CRISPR-Cas9 Genome Editing is a revolutionary method used in genetic engineering that enables precise modifications of DNA sequences in living organisms. This technology utilizes the Cas9 protein, which acts as molecular scissors to cut DNA at specific sites, guided by a sequence known as Protospacer or guide RNA. The significance of this method in business lies in its potential applications across various fields, including medicine, agriculture, and biotechnology, allowing companies to innovate and develop solutions for complex challenges.

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