Learning Path
Question & Answer1
Understand Question2
Review Options3
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Explore TopicChoose the Best Answer
A
Specific Taxonomic Identification
B
Complete Genome Reconstruction
C
Functional Gene Analysis
D
Environmental Sampling
Understanding the Answer
Let's break down why this is correct
Answer
Whole‑Genome Shotgun Metagenomic Sequencing gives a full, detailed view of all DNA in a sample, allowing researchers to see every gene and function. 16S Ribosomal RNA Gene Sequencing, by contrast, focuses on a single, highly conserved gene that is present in all bacteria and archaea. Because that gene varies enough to distinguish different species, 16S sequencing is used mainly to identify and count the microbes present in a community. In short, 16S rRNA gene sequencing provides microbial community profiling, telling you which organisms are there and in what relative amounts. For example, sequencing the 16S gene from a stool sample can reveal that 30 % of the bacteria are Lactobacillus, 25 % Bacteroides, and the rest are various other species.
Detailed Explanation
16S rRNA sequencing looks at a single, highly conserved gene that changes a little between species. Other options are incorrect because The misconception is that 16S sequencing can rebuild entire genomes; Some think 16S tells us about functions of genes.
Key Concepts
Whole-Genome Shotgun Metagenomic Sequencing
16S Ribosomal RNA Gene Sequencing
Microbial Diversity Analysis
Topic
Whole-Genome Shotgun Metagenomic Sequencing
Difficulty
medium level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1How does 16S Ribosomal RNA gene sequencing contribute to metagenomics and taxonomy through culture-independent methods?
hardBiological-sciences
Practice
2
Question 2What is the importance of DNA extraction techniques in Whole-Genome Shotgun Metagenomic Sequencing?
mediumBiological-sciences
Practice
3
Question 3Which bioinformatics tool is crucial for analyzing data from Whole-Genome Shotgun Metagenomic Sequencing and what are some future directions that this field might take to enhance microbial community understanding?
mediumBiological-sciences
Practice
4
Question 4What is the primary reason why whole-genome shotgun metagenomic sequencing is preferred over targeted sequencing methods in analyzing microbial communities?
mediumBiological-sciences
Practice
5
Question 5Which of the following statements best describes the significance of whole-genome shotgun metagenomic sequencing in studying microbial communities?
easyBiological-sciences
Practice
6
Question 6What is a primary advantage of whole-genome shotgun metagenomic sequencing over traditional sequencing methods?
mediumBiological-sciences
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7
Question 7Arrange the following steps in the process of Whole-Genome Shotgun Metagenomic Sequencing from start to finish: A) Sequence the DNA fragments, B) Extract DNA from the microbial sample, C) Assemble the sequenced fragments into a complete genome, D) Analyze the genetic data for species identification.
easyBiological-sciences
Practice
8
Question 8Which of the following statements accurately describe the advantages of using whole-genome shotgun metagenomic sequencing in studying microbial communities? Select all that apply.
hardBiological-sciences
Practice
9
Question 9Whole-genome shotgun metagenomic sequencing allows researchers to obtain a comprehensive view of the genetic content of a microbial community, including the ability to identify different __________ present in the sample.
easyBiological-sciences
Practice
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