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Question & Answer
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It allows for the identification of both known and unknown species.
It requires prior knowledge of the organisms present in the sample.
It provides insights into the functional capabilities of the microbial community.
It can sequence all DNA, including that from viruses and other non-bacterial entities.
It is limited to sequencing only a specific region of a genome.
Understanding the Answer
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Shotgun sequencing reads all DNA fragments, so it can spot species that scientists already know and new ones that were not seen before. Other options are incorrect because Some think you need to know the microbes first, but shotgun reads everything without guessing; Some think shotgun only tells who is there, not what they can do.
Key Concepts
Whole-Genome Shotgun Metagenomic Sequencing
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Deep Dive: Whole-Genome Shotgun Metagenomic Sequencing
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Definition
Whole-genome shotgun metagenomic sequencing is a technique that involves sequencing all DNA present in a microbial sample. This approach provides a comprehensive view of the genetic content of a microbial community, allowing for the identification of species and functional analysis.
Topic Definition
Whole-genome shotgun metagenomic sequencing is a technique that involves sequencing all DNA present in a microbial sample. This approach provides a comprehensive view of the genetic content of a microbial community, allowing for the identification of species and functional analysis.
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