Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
By analyzing only individual genomes in isolation.
B
By providing comprehensive data on all microbial DNA present, facilitating the study of microbial interactions and functions.
C
By focusing solely on eukaryotic microbes and ignoring prokaryotic organisms.
D
By limiting the analysis to a single environmental sample type.
Understanding the Answer
Let's break down why this is correct
Answer
Whole‑Genome Shotgun Metagenomic Sequencing (WGS‑MS) reads all DNA fragments from a sample, so it captures every organism’s genetic material, not just a few marker genes. By assembling these fragments, scientists can identify which species are present, estimate their relative abundance, and discover the genes they carry, revealing metabolic capabilities and interactions. Comparing WGS‑MS data across time or locations lets researchers see how microbial communities shift, such as how soil microbes change after a crop rotation or how gut bacteria respond to a new diet. For example, sequencing soil before and after a fertilizer application can show increases in nitrogen‑fixing bacteria, linking environmental change to functional shifts in the microbiome. This comprehensive view helps explain how microbes adapt, cooperate, or compete in different ecosystems.
Detailed Explanation
Whole‑Genome Shotgun Metagenomic Sequencing reads every piece of DNA in a sample. Other options are incorrect because Some think shotgun looks at one genome at a time; The method does not ignore bacteria or archaea.
Key Concepts
Metagenomics
Microbiome analysis
Environmental genomics
Topic
Whole-Genome Shotgun Metagenomic Sequencing
Difficulty
hard level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1What is the importance of DNA extraction techniques in Whole-Genome Shotgun Metagenomic Sequencing?
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Question 2Which bioinformatics tool is crucial for analyzing data from Whole-Genome Shotgun Metagenomic Sequencing and what are some future directions that this field might take to enhance microbial community understanding?
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3
Question 3What is the primary reason why whole-genome shotgun metagenomic sequencing is preferred over targeted sequencing methods in analyzing microbial communities?
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Question 4Which of the following statements best describes the significance of whole-genome shotgun metagenomic sequencing in studying microbial communities?
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5
Question 5What is a primary advantage of whole-genome shotgun metagenomic sequencing over traditional sequencing methods?
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6
Question 6How does whole-genome shotgun metagenomic sequencing enhance our understanding of microbial communities in the human microbiome?
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7
Question 7Which of the following statements accurately describe the advantages of using whole-genome shotgun metagenomic sequencing in studying microbial communities? Select all that apply.
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8
Question 8Whole-genome shotgun metagenomic sequencing allows researchers to obtain a comprehensive view of the genetic content of a microbial community, including the ability to identify different __________ present in the sample.
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