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A standard curve is created by plotting known concentrations of a protein against their corresponding absorbance values.
Standard curves can only be used for quantifying proteins in pure solutions, not in complex mixtures.
The slope of the standard curve indicates the sensitivity of the measurement method.
Standard curve generation is unnecessary if the absorbance readings are within a certain range.
A standard curve can help determine the concentration of an unknown protein sample when compared to known standards.
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Standard Curve Generation
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