Order the steps involved in generating a standard curve for protein measurement using the Folin-Ciocalteu reagent.

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Prepare a series of known protein concentrations → B. Measure absorbance of each concentration → C. Plot absorbance values against concentrations → D. Use the standard curve to determine unknown concentrations

Prepare a series of known protein concentrations → C. Plot absorbance values against concentrations → A. Measure absorbance of each concentration → D. Use the standard curve to determine unknown concentrations

Measure absorbance of each concentration → A. Prepare a series of known protein concentrations → D. Use the standard curve to determine unknown concentrations → B. Plot absorbance values against concentrations

Use the standard curve to determine unknown concentrations → A. Prepare a series of known protein concentrations → B. Measure absorbance of each concentration → C. Plot absorbance values against concentrations

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Answer

To create a standard curve you first make a series of protein solutions whose concentrations you know exactly, such as 0, 0. 5, 1. 0, 1. 5 and 2. 0 mg mL⁻¹.

Detailed Explanation

First, you create several tubes with known amounts of protein. Other options are incorrect because This answer puts the graph before the measurements, which is impossible because you need data first; It starts by measuring, but you cannot measure without knowing the amounts first.

Key Concepts

Standard Curve Generation

Protein Measurement Techniques

Colorimetric Analysis

Topic

Standard Curve Generation

Difficulty

hard level question

Cognitive Level

understand

Practice Similar Questions

Test your understanding with related questions

Question 1

The __________ method is commonly used for measuring protein concentration in solutions and is based on the reaction of proteins with the Folin-Ciocalteu reagent.

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Question 2

Folin-Ciocalteu reagent : protein quantification :: Bradford assay : ?

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Question 3

Order the steps involved in utilizing the Folin phenol reagent for protein quantification, starting from the sample preparation to the final measurement.

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Question 4

Which of the following statements about standard curve generation in protein measurement are true? (Select all that apply)

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Question 5

A researcher is attempting to determine the concentration of a protein in an unknown sample using the Folin-Ciocalteu method. They prepare a standard curve using known concentrations of a standard protein solution and measure the corresponding absorbance values. What is the most critical factor the researcher must ensure when generating the standard curve for accurate quantification of the unknown sample?

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