In standard curve generation for protein measurement, the relationship between ________ and absorbance values is crucial for accurate quantification of unknown samples.

In standard curve generation for protein measurement, the relationship between concentration and absorbance values is crucial for accurate quantification of unknown samples. The standard curve plots known protein concentrations on the horizontal axis against their measured absorbance on the vertical axis, creating a line that represents how absorbance increases with concentration. By fitting a straight line (or sometimes a curve) through these points, we obtain an equation that translates any absorbance reading into a protein concentration. For example, if a 10 µg/mL standard gives an absorbance of 0. 50, the curve lets us determine that a sample with absorbance 0.