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Explore TopicChoose the Best Answer
A
True
B
False
Understanding the Answer
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Answer
True. A standard curve shows how the color intensity relates to known protein amounts, so you can read off the concentration of an unknown sample only if its color falls inside that curve’s range. If the unknown’s color is outside the curve, you can’t accurately match it to a concentration. Therefore, you must dilute the sample so its absorbance falls within the standard curve’s limits. Once it’s inside the range, the curve gives a reliable protein concentration.
Detailed Explanation
The curve shows how absorbance changes with protein amount. Other options are incorrect because Some think any absorbance can be used.
Key Concepts
Standard Curve Generation
Protein Measurement Techniques
Colorimetric Analysis
Topic
Standard Curve Generation
Difficulty
medium level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1How does a standard curve aid in the accurate measurement of protein concentration?
easyBiological-sciences
Practice
2
Question 2If a standard curve generated from known protein concentrations shows a drastic deviation from expected absorbance values for unknown samples, what could be a potential underlying cause?
mediumBiological-sciences
Practice
3
Question 3In standard curve generation for protein measurement, the relationship between ________ and absorbance values is crucial for accurate quantification of unknown samples.
mediumBiological-sciences
Practice
4
Question 4A researcher is attempting to determine the concentration of a protein in an unknown sample using the Folin-Ciocalteu method. They prepare a standard curve using known concentrations of a standard protein solution and measure the corresponding absorbance values. What is the most critical factor the researcher must ensure when generating the standard curve for accurate quantification of the unknown sample?
mediumBiological-sciences
Practice
5
Question 5A researcher is attempting to measure the protein concentration in an unknown sample using a standard curve generated from known concentrations of BSA (Bovine Serum Albumin). Which of the following factors is most critical for ensuring the accuracy of the protein quantification from the standard curve?
hardBiological-sciences
Practice
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