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Question & Answer1
Understand Question2
Review Options3
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Explore TopicChoose the Best Answer
A
Sample preparation is unnecessary when using spectrophotometry for protein measurement.
B
Proper sample preparation can increase the accuracy of spectrophotometric measurements by removing interfering substances.
C
Sample preparation is only relevant for qualitative analysis and not for quantitative spectrophotometry.
D
All proteins can be measured directly without any form of sample preparation.
Understanding the Answer
Let's break down why this is correct
Answer
Sample preparation in spectrophotometry for proteins is essential because it removes substances that can absorb light at the same wavelength as the protein, such as nucleic acids or detergents, and it brings the protein concentration into the linear range of the instrument. First, the protein is purified or diluted so that its absorbance falls between 0. 1 and 1. 0, which is the most reliable part of the Beer–Lambert law. Next, the sample is clarified, often by centrifugation or filtration, to eliminate particulates that scatter light and distort the reading.
Detailed Explanation
When you clean a sample, you remove things that can hide or change the light signal. Other options are incorrect because Some think you can skip cleaning, but leftover salts or dyes still affect the light; Quantitative tests need exact numbers, not just a yes or no.
Key Concepts
Spectrophotometry
Sample preparation
Topic
Protein Measurement Methods
Difficulty
medium level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1In the context of protein measurement methods, which of the following best describes the difference between accuracy and precision?
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2
Question 2Which of the following methods can be used to measure protein concentration in a sample, utilizing the principles of both spectrophotometry and enzymatic assays?
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3
Question 3Which of the following statements about standard curve generation in protein measurement are true? (Select all that apply)
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4
Question 4Which of the following scenarios best illustrates the process of protein denaturation and its impact on protein measurement methods?
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