Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
Biuret method
B
Lowry method
C
Bradford assay
D
BCA method
Understanding the Answer
Let's break down why this is correct
Answer
The method that uses a colorimetric reaction with phenolic compounds is the Lowry protein assay. It relies on the Folin‑Ciocalteu reagent, which reacts with phenolic and some other amino‑acid side chains in the protein to produce a blue color. The intensity of that color, measured spectrophotometrically, is proportional to the protein concentration. For example, if you add the reagent to a 1 µg/mL protein solution, the resulting blue hue can be quantified at 750 nm to determine the exact amount of protein present. This approach is more sensitive than the Bradford assay but requires careful handling of the reagent.
Detailed Explanation
The Folin-Ciocalteu reagent reacts with phenolic groups in proteins. Other options are incorrect because This method uses copper ions that bind to peptide bonds, not phenolic groups; This assay uses a dye that sticks to proteins, not phenolic groups.
Key Concepts
Protein measurement methods
Colorimetric assays
Quantitative analysis
Topic
Protein Measurement Methods
Difficulty
easy level question
Cognitive Level
understand
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