📚 Learning Guide
Protein Measurement Methods
easy

Which of the following protein measurement methods relies on colorimetric reaction with phenolic compounds to quantify protein content?

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Learning Path

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Choose the Best Answer

A

Biuret method

B

Lowry method

C

Bradford assay

D

BCA method

Understanding the Answer

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Answer

The method that uses a colorimetric reaction with phenolic compounds is the Lowry protein assay. It relies on the Folin‑Ciocalteu reagent, which reacts with phenolic and some other amino‑acid side chains in the protein to produce a blue color. The intensity of that color, measured spectrophotometrically, is proportional to the protein concentration. For example, if you add the reagent to a 1 µg/mL protein solution, the resulting blue hue can be quantified at 750 nm to determine the exact amount of protein present. This approach is more sensitive than the Bradford assay but requires careful handling of the reagent.

Detailed Explanation

The Folin-Ciocalteu reagent reacts with phenolic groups in proteins. Other options are incorrect because This method uses copper ions that bind to peptide bonds, not phenolic groups; This assay uses a dye that sticks to proteins, not phenolic groups.

Key Concepts

Protein measurement methods
Colorimetric assays
Quantitative analysis
Topic

Protein Measurement Methods

Difficulty

easy level question

Cognitive Level

understand

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