📚 Learning Guide
Protein Measurement Methods
easy

Which of the following methods can be used for measuring protein concentration in biological samples? (Select all that apply)

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Learning Path
Learning Path

Question & Answer
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2
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3
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4
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Choose the Best Answer

A

Bradford Assay

B

BCA Assay

C

Gel Electrophoresis

D

Spectrophotometry

E

PCR amplification

Understanding the Answer

Let's break down why this is correct

Answer

Protein concentration in biological samples can be measured with several common laboratory methods. The Bradford assay uses a dye that binds to proteins, the Lowry method relies on a color change after reacting with copper ions, and the bicinchoninic acid (BCA) assay also produces a colored product that can be read by a spectrophotometer. A simple UV absorption measurement at 280 nm takes advantage of the fact that aromatic amino acids absorb light at this wavelength, allowing a quick estimate of protein amount. Finally, an enzyme‑linked immunosorbent assay (ELISA) can quantify specific proteins with high sensitivity. Each of these approaches works well for different sample types and desired accuracy.

Detailed Explanation

Bradford and BCA assays use dyes that change color when they bind proteins. Other options are incorrect because Gel Electrophoresis separates proteins by size, not by amount; PCR makes many copies of DNA, not proteins.

Key Concepts

Protein quantification methods
Biological assays
Spectrophotometry
Topic

Protein Measurement Methods

Difficulty

easy level question

Cognitive Level

understand

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