Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
Bradford Assay
B
BCA Assay
C
Gel Electrophoresis
D
Spectrophotometry
E
PCR amplification
Understanding the Answer
Let's break down why this is correct
Answer
Protein concentration in biological samples can be measured with several common laboratory methods. The Bradford assay uses a dye that binds to proteins, the Lowry method relies on a color change after reacting with copper ions, and the bicinchoninic acid (BCA) assay also produces a colored product that can be read by a spectrophotometer. A simple UV absorption measurement at 280 nm takes advantage of the fact that aromatic amino acids absorb light at this wavelength, allowing a quick estimate of protein amount. Finally, an enzyme‑linked immunosorbent assay (ELISA) can quantify specific proteins with high sensitivity. Each of these approaches works well for different sample types and desired accuracy.
Detailed Explanation
Bradford and BCA assays use dyes that change color when they bind proteins. Other options are incorrect because Gel Electrophoresis separates proteins by size, not by amount; PCR makes many copies of DNA, not proteins.
Key Concepts
Protein quantification methods
Biological assays
Spectrophotometry
Topic
Protein Measurement Methods
Difficulty
easy level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1Which of the following methods can be used to measure protein concentration in a sample, utilizing the principles of both spectrophotometry and enzymatic assays?
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2
Question 2In the context of protein measurement methods, how does the Bradford assay utilize calibration curves during sample preparation to determine protein concentration?
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3
Question 3Which method is most suitable for accurately measuring low concentrations of proteins in a sample?
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4
Question 4Which protein measurement method is most suitable for detecting low concentrations of proteins in a sample?
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5
Question 5What is the correct sequence of steps for measuring protein concentration using an alkaline copper solution in a colorimetric assay?
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