Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
Bradford
B
Lowry
C
Biuret
D
BCA
Understanding the Answer
Let's break down why this is correct
Answer
The Lowry method is commonly used for measuring protein concentration in solutions and is based on the reaction of proteins with the Folin‑Ciocalteu reagent. In this assay, proteins reduce the reagent, producing a blue color that is measured at 750 nm. The intensity of the blue color is proportional to the protein amount, so a standard curve with known protein concentrations is used for quantification. For example, if a sample yields a color intensity that matches a 1 mg/mL standard, the sample is determined to contain 1 mg/mL of protein. This method is popular because it is relatively sensitive and can be used with many different protein types.
Detailed Explanation
The Lowry method uses the Folin-Ciocalteu reagent, which reacts with protein to form a blue color. Other options are incorrect because Bradford uses a blue dye that binds to arginine residues, not Folin-Ciocalteu; Biuret uses copper ions that react with peptide bonds to give a purple color.
Key Concepts
Protein Measurement Techniques
Quantification Methods
Colorimetric Assays
Topic
Protein Measurement Methods
Difficulty
easy level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1Order the steps involved in generating a standard curve for protein measurement using the Folin-Ciocalteu reagent.
hardBiological-sciences
Practice
2
Question 2A researcher is attempting to determine the concentration of a protein in an unknown sample using the Folin-Ciocalteu method. They prepare a standard curve using known concentrations of a standard protein solution and measure the corresponding absorbance values. What is the most critical factor the researcher must ensure when generating the standard curve for accurate quantification of the unknown sample?
mediumBiological-sciences
Practice
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