📚 Learning Guide
Protein Measurement Methods
easy

The __________ method is commonly used for measuring protein concentration in solutions and is based on the reaction of proteins with the Folin-Ciocalteu reagent.

Master this concept with our detailed explanation and step-by-step learning approach

Learning Path
Learning Path

Question & Answer
1
Understand Question
2
Review Options
3
Learn Explanation
4
Explore Topic

Choose the Best Answer

A

Bradford

B

Lowry

C

Biuret

D

BCA

Understanding the Answer

Let's break down why this is correct

Answer

The Lowry method is commonly used for measuring protein concentration in solutions and is based on the reaction of proteins with the Folin‑Ciocalteu reagent. In this assay, proteins reduce the reagent, producing a blue color that is measured at 750 nm. The intensity of the blue color is proportional to the protein amount, so a standard curve with known protein concentrations is used for quantification. For example, if a sample yields a color intensity that matches a 1 mg/mL standard, the sample is determined to contain 1 mg/mL of protein. This method is popular because it is relatively sensitive and can be used with many different protein types.

Detailed Explanation

The Lowry method uses the Folin-Ciocalteu reagent, which reacts with protein to form a blue color. Other options are incorrect because Bradford uses a blue dye that binds to arginine residues, not Folin-Ciocalteu; Biuret uses copper ions that react with peptide bonds to give a purple color.

Key Concepts

Protein Measurement Techniques
Quantification Methods
Colorimetric Assays
Topic

Protein Measurement Methods

Difficulty

easy level question

Cognitive Level

understand

Ready to Master More Topics?

Join thousands of students using Seekh's interactive learning platform to excel in their studies with personalized practice and detailed explanations.