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Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
It is used to determine the concentration of a protein sample based on its absorbance at 595 nm.
B
It is not applicable to spectrophotometric methods.
C
It only applies to low molecular weight proteins.
D
It cannot be used for quantitative analysis.
Understanding the Answer
Let's break down why this is correct
Answer
In the Bradford assay, the dye binds protein and shifts the dye’s absorption maximum to 595 nm, so a spectrophotometer measures the resulting absorbance. By running several known protein standards, a calibration curve of absorbance versus protein concentration is built, which follows Beer‑Lambert’s law. This curve is then used to interpolate the concentration of an unknown sample from its measured absorbance. For example, if a sample shows an absorbance of 0. 45 AU, the curve might indicate it contains 2 mg/mL of protein.
Detailed Explanation
The Bradford assay uses a dye that binds protein and changes color. Other options are incorrect because Some think Bradford is not spectrophotometric, but it relies on light absorption; The assay works for many proteins, not just low‑molecular‑weight ones.
Key Concepts
Spectrophotometry
Bradford assay
Calibration curves
Topic
Protein Measurement Methods
Difficulty
hard level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1In the context of protein measurement methods, how does the Bradford assay utilize calibration curves during sample preparation to determine protein concentration?
hardBiological-sciences
Practice
2
Question 2Which of the following statements about standard curve generation in protein measurement are true? (Select all that apply)
easyBiological-sciences
Practice
3
Question 3In standard curve generation for protein measurement, the relationship between ________ and absorbance values is crucial for accurate quantification of unknown samples.
mediumBiological-sciences
Practice
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