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It is used to determine the concentration of a protein sample based on its absorbance at 595 nm.
It is not applicable to spectrophotometric methods.
It only applies to low molecular weight proteins.
It cannot be used for quantitative analysis.
Understanding the Answer
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The Bradford assay uses a dye that binds protein and changes color. Other options are incorrect because Some think Bradford is not spectrophotometric, but it relies on light absorption; The assay works for many proteins, not just low‑molecular‑weight ones.
Key Concepts
Protein Measurement Methods
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Deep Dive: Protein Measurement Methods
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Definition
Protein measurement methods involve various techniques and reagents to quantify protein content in biological samples. This includes procedures for measuring proteins in solution, after precipitation, and determining minimal protein concentrations. Understanding the principles and limitations of protein measurement methods is crucial for accurate analysis.
Topic Definition
Protein measurement methods involve various techniques and reagents to quantify protein content in biological samples. This includes procedures for measuring proteins in solution, after precipitation, and determining minimal protein concentrations. Understanding the principles and limitations of protein measurement methods is crucial for accurate analysis.
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