In the context of protein measurement methods, how does the Bradford assay utilize calibration curves during sample preparation to determine protein concentration?

The Bradford assay measures protein by how much a dye binds, causing a color change that can be read with a spectrophotometer. To translate the measured absorbance into a protein concentration, the lab first runs a set of known protein standards and records their absorbances, creating a calibration curve that plots absorbance versus protein amount. When a sample is added to the dye, its absorbance is measured and plotted on this curve; the corresponding protein concentration is read off from the curve’s interpolation. For example, if a 10 µg/mL standard gives an absorbance of 0. 5 and a sample shows 0.