Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
DNA quantification
B
Lipid quantification
C
Protein quantification
D
Enzyme activity measurement
Understanding the Answer
Let's break down why this is correct
Answer
Folin‑Ciocalteu reagent is used to determine how much phenolic compounds are in a sample. In the same way, the Bradford assay is a quick color‑change test that tells you how much protein is present. Both methods rely on a dye that shifts color when it binds to its target molecules. For example, adding Bradford dye to a protein solution turns it blue, and the deeper the blue, the more protein is there. Thus, the Bradford assay is to protein measurement what Folin‑Ciocalteu is to phenolics.
Detailed Explanation
Bradford uses a dye that binds to arginine and other basic amino acids in proteins. Other options are incorrect because Some think Bradford measures DNA because it changes color; Bradford does not detect fats.
Key Concepts
Protein quantification methods
Analytical chemistry techniques
Spectrophotometry
Topic
Protein Measurement Methods
Difficulty
medium level question
Cognitive Level
understand
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