📚 Learning Guide
Protein Measurement Methods
medium

Folin-Ciocalteu reagent : protein quantification :: Bradford assay : ?

Master this concept with our detailed explanation and step-by-step learning approach

Learning Path
Learning Path

Question & Answer
1
Understand Question
2
Review Options
3
Learn Explanation
4
Explore Topic

Choose the Best Answer

A

DNA quantification

B

Lipid quantification

C

Protein quantification

D

Enzyme activity measurement

Understanding the Answer

Let's break down why this is correct

Answer

Folin‑Ciocalteu reagent is used to determine how much phenolic compounds are in a sample. In the same way, the Bradford assay is a quick color‑change test that tells you how much protein is present. Both methods rely on a dye that shifts color when it binds to its target molecules. For example, adding Bradford dye to a protein solution turns it blue, and the deeper the blue, the more protein is there. Thus, the Bradford assay is to protein measurement what Folin‑Ciocalteu is to phenolics.

Detailed Explanation

Bradford uses a dye that binds to arginine and other basic amino acids in proteins. Other options are incorrect because Some think Bradford measures DNA because it changes color; Bradford does not detect fats.

Key Concepts

Protein quantification methods
Analytical chemistry techniques
Spectrophotometry
Topic

Protein Measurement Methods

Difficulty

medium level question

Cognitive Level

understand

Ready to Master More Topics?

Join thousands of students using Seekh's interactive learning platform to excel in their studies with personalized practice and detailed explanations.