Learning Path
Question & Answer
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Add Biuret reagent to the protein solution.
Measure the absorbance at 540 nm.
Prepare a standard curve using known protein concentrations.
Incubate the reaction mixture at room temperature.
Understanding the Answer
Let's break down why this is correct
First, a standard curve is made so we know how absorbance relates to protein amount. Other options are incorrect because Adding the reagent first skips the standard curve, so we have no reference for the unknown sample; Measuring absorbance before the color has time to form gives a low reading.
Key Concepts
Protein Measurement Methods
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Deep Dive: Protein Measurement Methods
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Definition
Protein measurement methods involve various techniques and reagents to quantify protein content in biological samples. This includes procedures for measuring proteins in solution, after precipitation, and determining minimal protein concentrations. Understanding the principles and limitations of protein measurement methods is crucial for accurate analysis.
Topic Definition
Protein measurement methods involve various techniques and reagents to quantify protein content in biological samples. This includes procedures for measuring proteins in solution, after precipitation, and determining minimal protein concentrations. Understanding the principles and limitations of protein measurement methods is crucial for accurate analysis.
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