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Exposure to extreme pH → Disruption of hydrogen bonds → Loss of secondary structure → Denatured protein
Loss of secondary structure → Disruption of hydrogen bonds → Exposure to extreme pH → Denatured protein
Disruption of hydrogen bonds → Exposure to extreme pH → Loss of secondary structure → Denatured protein
Denatured protein → Loss of secondary structure → Exposure to extreme pH → Disruption of hydrogen bonds
Understanding the Answer
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When the pH is very high or very low, the charged groups on the protein change. Other options are incorrect because It says the protein loses its shape before the pH changes; It claims the bonds break before the pH changes.
Key Concepts
Protein Denaturation
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Deep Dive: Protein Denaturation
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Definition
Protein denaturation poses challenges in protein measurement methods, affecting the accuracy and reliability of quantification results. Understanding the factors leading to protein denaturation, such as surface denaturation in bovine albumin solutions, is crucial for optimizing sample preparation and ensuring precise protein analysis.
Topic Definition
Protein denaturation poses challenges in protein measurement methods, affecting the accuracy and reliability of quantification results. Understanding the factors leading to protein denaturation, such as surface denaturation in bovine albumin solutions, is crucial for optimizing sample preparation and ensuring precise protein analysis.
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