Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
pH changes
B
temperature fluctuations
C
ionic strength variations
D
all of the above
Understanding the Answer
Let's break down why this is correct
Answer
Surface denaturation in bovine albumin solutions lets us estimate protein concentration by measuring the turbidity that appears when proteins unfold on a surface. In enzyme work the parallel is the rate of substrate turnover, which tells us how fast the enzyme converts substrate to product. Both methods rely on a measurable change that is directly proportional to the amount of protein or enzyme present. Thus, surface denaturation is to protein measurement what substrate turnover is to enzyme activity.
Detailed Explanation
All three factors—acidity, heat, and salt level—can disturb enzyme activity just as surface denaturation affects protein measurement. Other options are incorrect because Thinking only acidity matters ignores that heat and salt also affect enzymes; Believing only heat matters overlooks acidity and salt.
Key Concepts
Protein denaturation
Enzyme activity
Biochemical measurement
Topic
Protein Denaturation
Difficulty
hard level question
Cognitive Level
understand
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