A researcher is preparing a sample of bovine serum albumin (BSA) for a protein quantification assay. During the preparation, they accidentally expose the sample to high temperatures and strong agitation. What is the most likely consequence of these conditions on the BSA sample, and how should the researcher proceed to ensure accurate results?

High temperatures and vigorous shaking will cause the BSA to unfold and aggregate, destroying its native structure and exposing hydrophobic patches that stick together. This denatured protein no longer behaves the same in a quantification assay, so the measured absorbance or color change will be inaccurate. The researcher should discard the damaged aliquot and prepare a fresh, properly stored sample, keeping it on ice and mixing gently. If the protein must be reused, the researcher could try to refold it by slowly cooling it and adding a suitable buffer, but the safest approach is to start over with a new, properly handled BSA stock. This ensures the assay reflects the true protein concentration.