Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
It reacts specifically with nucleic acids
B
It is highly sensitive to proteins
C
It is stable without requiring refrigeration
D
It can measure carbohydrates effectively
Understanding the Answer
Let's break down why this is correct
Answer
The Folin phenol reagent reacts with the aromatic amino acids, especially tyrosine and tryptophan, in a protein sample, producing a blue color that can be measured spectrophotometrically. This color change is directly proportional to the amount of protein present, giving a reliable way to quantify protein concentration. Because the reagent is sensitive to a wide range of protein types and works well with many buffers, it is widely used in the Bradford assay, which is fast, inexpensive, and compatible with most laboratory settings. For example, adding the reagent to a sample of bovine serum albumin and measuring absorbance at 595 nm allows a researcher to determine that the sample contains 0. 5 mg mL⁻¹ protein.
Detailed Explanation
The reagent reacts with phenolic groups in proteins, forming a blue color. Other options are incorrect because Some think the reagent works mainly with nucleic acids, but it actually reacts with phenolic groups in proteins; People sometimes say the reagent is stable without refrigeration, but it still needs cool, dry storage to keep its chemicals balanced.
Key Concepts
Protein quantification
Analytical chemistry
Biological samples
Topic
Folin Phenol Reagent
Difficulty
easy level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1In pharmaceutical applications, how does the preparation of Folin Phenol Reagent influence laboratory procedures during the analysis of phenolic compounds?
hardBiological-sciences
Practice
2
Question 2Why is the Folin phenol reagent preferred for protein measurement over other reagents?
mediumBiological-sciences
Practice
3
Question 3The effectiveness of protein measurement using the Folin phenol reagent is largely dependent on its interaction with __________, which can lead to inaccurate results if not properly managed.
hardBiological-sciences
Practice
4
Question 4A researcher is conducting an experiment to quantify protein concentration in a series of biological samples. They decide to use the Folin phenol reagent due to its sensitivity and simplicity. What is the most critical consideration the researcher should keep in mind when using this reagent to ensure accurate results?
easyBiological-sciences
Practice
5
Question 5Folin Phenol Reagent:Protein Measurement :: Biuret Reagent: ?
hardBiological-sciences
Practice
6
Question 6Order the steps involved in utilizing the Folin phenol reagent for protein quantification, starting from the sample preparation to the final measurement.
mediumBiological-sciences
Practice
7
Question 7If the Folin phenol reagent produces a color change when mixed with a protein sample, what underlying mechanism explains this observation?
easyBiological-sciences
Practice
8
Question 8Which of the following scenarios best illustrates the appropriate application of the Folin phenol reagent in protein quantification?
mediumBiological-sciences
Practice
9
Question 9How does protein denaturation impact the accuracy of protein quantification methods like the Folin phenol reagent?
mediumBiological-sciences
Practice
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