📚 Learning Guide
Folin Phenol Reagent
easy

Which of the following statements about the Folin phenol reagent are true? (Select all that apply)

Master this concept with our detailed explanation and step-by-step learning approach

Learning Path
Learning Path

Question & Answer
1
Understand Question
2
Review Options
3
Learn Explanation
4
Explore Topic

Choose the Best Answer

A

The Folin phenol reagent is primarily used for DNA quantification.

B

The reagent is sensitive and used for measuring protein concentration.

C

The Folin phenol reagent reacts with phenolic compounds to produce a color change.

D

It requires complex preparation and handling to be effective.

E

The reagent can be used in various biological sample analyses.

Understanding the Answer

Let's break down why this is correct

Answer

The Folin‑Ciocalteu reagent is a mixture of phosphomolybdic and phosphotungstic acids that, when mixed with an alkaline solution, reacts with phenolic compounds to produce a blue‑violet color whose intensity is proportional to the phenolic content. The reaction requires a strongly alkaline medium, usually sodium carbonate, to deprotonate the phenols and allow reduction of the metal oxides. Because the reagent is sensitive to any reducing agent, not only phenols but also sugars, ascorbic acid and some amino acids can also produce a blue color, so the test is not entirely specific. In practice, a small cup of tea is added to the reagent and the resulting blue hue is compared to a standard curve of gallic acid to estimate total phenolics. Thus, the reagent is useful for estimating total phenolic content but must be interpreted with caution due to possible interference.

Detailed Explanation

The Folin phenol reagent is a sensitive test that measures protein concentration, so option B is right. Other options are incorrect because Some people think it is used for DNA, but DNA needs dyes like PicoGreen; The reagent is easy to use.

Key Concepts

Protein quantification
Chemical reactions of reagents
Analytical methods in biology
Topic

Folin Phenol Reagent

Difficulty

easy level question

Cognitive Level

understand

Ready to Master More Topics?

Join thousands of students using Seekh's interactive learning platform to excel in their studies with personalized practice and detailed explanations.