Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
The Folin phenol reagent is primarily used for DNA quantification.
B
The reagent is sensitive and used for measuring protein concentration.
C
The Folin phenol reagent reacts with phenolic compounds to produce a color change.
D
It requires complex preparation and handling to be effective.
E
The reagent can be used in various biological sample analyses.
Understanding the Answer
Let's break down why this is correct
Answer
The Folin‑Ciocalteu reagent is a mixture of phosphomolybdic and phosphotungstic acids that, when mixed with an alkaline solution, reacts with phenolic compounds to produce a blue‑violet color whose intensity is proportional to the phenolic content. The reaction requires a strongly alkaline medium, usually sodium carbonate, to deprotonate the phenols and allow reduction of the metal oxides. Because the reagent is sensitive to any reducing agent, not only phenols but also sugars, ascorbic acid and some amino acids can also produce a blue color, so the test is not entirely specific. In practice, a small cup of tea is added to the reagent and the resulting blue hue is compared to a standard curve of gallic acid to estimate total phenolics. Thus, the reagent is useful for estimating total phenolic content but must be interpreted with caution due to possible interference.
Detailed Explanation
The Folin phenol reagent is a sensitive test that measures protein concentration, so option B is right. Other options are incorrect because Some people think it is used for DNA, but DNA needs dyes like PicoGreen; The reagent is easy to use.
Key Concepts
Protein quantification
Chemical reactions of reagents
Analytical methods in biology
Topic
Folin Phenol Reagent
Difficulty
easy level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1What role does Folin Phenol Reagent play in the analysis of wine?
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2
Question 2In pharmaceutical applications, how does the preparation of Folin Phenol Reagent influence laboratory procedures during the analysis of phenolic compounds?
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3
Question 3Why is the Folin phenol reagent particularly important in protein measurement?
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4
Question 4Which of the following scenarios best illustrates the appropriate application of the Folin phenol reagent in protein quantification?
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