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Folin Phenol Reagent
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Which of the following scenarios best illustrates the appropriate application of the Folin phenol reagent in protein quantification?

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A

Measuring the protein concentration in a plant extract to compare with animal tissues

B

Analyzing the amount of glucose in a serum sample

C

Determining the total RNA content in a bacterial culture

D

Assessing the lipid concentration in a cell membrane preparation

Understanding the Answer

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The Folin reagent reacts with the amino acids in proteins, forming a blue color that can be measured. Other options are incorrect because Glucose does not trigger the Folin reaction; RNA does not contain the amino acids that the Folin reagent needs.

Key Concepts

Protein quantification
Analytical chemistry techniques
Biological sample analysis
Topic

Folin Phenol Reagent

Difficulty

medium level question

Cognitive Level

understand

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Definition
Definition

The Folin phenol reagent is a key component in protein measurement methods, known for its sensitivity and simplicity. This reagent is utilized in various analytical procedures to detect proteins in different biological samples. Understanding the composition, stability, and reactions of the Folin phenol reagent is essential for accurate protein quantification.

Topic Definition

The Folin phenol reagent is a key component in protein measurement methods, known for its sensitivity and simplicity. This reagent is utilized in various analytical procedures to detect proteins in different biological samples. Understanding the composition, stability, and reactions of the Folin phenol reagent is essential for accurate protein quantification.

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