Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
Prepare the protein sample → B. Add Folin phenol reagent → C. Measure absorbance → D. Compare with standard curve
B
Prepare the protein sample → C. Measure absorbance → D. Add Folin phenol reagent → A. Compare with standard curve
C
Add Folin phenol reagent → D. Measure absorbance → A. Prepare the protein sample → B. Compare with standard curve
D
Measure absorbance → A. Compare with standard curve → B. Prepare the protein sample → C. Add Folin phenol reagent
Understanding the Answer
Let's break down why this is correct
Answer
First, prepare your protein sample by diluting it in a suitable buffer so the concentration falls within the assay’s range, then mix the diluted sample with a known volume of Folin‑phenol reagent. After adding the reagent, stir gently and let the reaction sit at room temperature for about 30 minutes to allow the blue color to develop. Next, transfer the mixture to a cuvette or microplate well and measure its absorbance at 750 nm using a spectrophotometer. Finally, compare this absorbance to a standard curve made from known protein concentrations to calculate the protein amount in your sample. For example, if you diluted bovine serum albumin to 0.
Detailed Explanation
First you make the protein sample ready so it can react. Other options are incorrect because This answer mixes up the order; It starts with adding the reagent before the sample is ready, so the reaction would be wrong or messy.
Key Concepts
Protein Measurement
Analytical Chemistry
Spectrophotometry
Topic
Folin Phenol Reagent
Difficulty
medium level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1In pharmaceutical applications, how does the preparation of Folin Phenol Reagent influence laboratory procedures during the analysis of phenolic compounds?
hardBiological-sciences
Practice
2
Question 2Why is the Folin phenol reagent particularly important in protein measurement?
easyBiological-sciences
Practice
3
Question 3Why is the Folin phenol reagent preferred for protein measurement over other reagents?
mediumBiological-sciences
Practice
4
Question 4The effectiveness of protein measurement using the Folin phenol reagent is largely dependent on its interaction with __________, which can lead to inaccurate results if not properly managed.
hardBiological-sciences
Practice
5
Question 5A researcher is conducting an experiment to quantify protein concentration in a series of biological samples. They decide to use the Folin phenol reagent due to its sensitivity and simplicity. What is the most critical consideration the researcher should keep in mind when using this reagent to ensure accurate results?
easyBiological-sciences
Practice
6
Question 6Folin Phenol Reagent:Protein Measurement :: Biuret Reagent: ?
hardBiological-sciences
Practice
7
Question 7Which of the following scenarios best illustrates the appropriate application of the Folin phenol reagent in protein quantification?
mediumBiological-sciences
Practice
8
Question 8Order the steps involved in generating a standard curve for protein measurement using the Folin-Ciocalteu reagent.
hardBiological-sciences
Practice
9
Question 9How does protein denaturation impact the accuracy of protein quantification methods like the Folin phenol reagent?
mediumBiological-sciences
Practice
Ready to Master More Topics?
Join thousands of students using Seekh's interactive learning platform to excel in their studies with personalized practice and detailed explanations.