📚 Learning Guide
Folin Phenol Reagent
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Order the steps involved in utilizing the Folin phenol reagent for protein quantification, starting from the sample preparation to the final measurement.

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Learning Path
Learning Path

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Choose AnswerChoose the Best Answer

A

Prepare the protein sample → B. Add Folin phenol reagent → C. Measure absorbance → D. Compare with standard curve

B

Prepare the protein sample → C. Measure absorbance → D. Add Folin phenol reagent → A. Compare with standard curve

C

Add Folin phenol reagent → D. Measure absorbance → A. Prepare the protein sample → B. Compare with standard curve

D

Measure absorbance → A. Compare with standard curve → B. Prepare the protein sample → C. Add Folin phenol reagent

Understanding the Answer

Let's break down why this is correct

First you make the protein sample ready so it can react. Other options are incorrect because This answer mixes up the order; It starts with adding the reagent before the sample is ready, so the reaction would be wrong or messy.

Key Concepts

Protein Measurement
Analytical Chemistry
Spectrophotometry
Topic

Folin Phenol Reagent

Difficulty

medium level question

Cognitive Level

understand

Deep Dive: Folin Phenol Reagent

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Definition
Definition

The Folin phenol reagent is a key component in protein measurement methods, known for its sensitivity and simplicity. This reagent is utilized in various analytical procedures to detect proteins in different biological samples. Understanding the composition, stability, and reactions of the Folin phenol reagent is essential for accurate protein quantification.

Topic Definition

The Folin phenol reagent is a key component in protein measurement methods, known for its sensitivity and simplicity. This reagent is utilized in various analytical procedures to detect proteins in different biological samples. Understanding the composition, stability, and reactions of the Folin phenol reagent is essential for accurate protein quantification.

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