Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
DNA Quantification
B
Lipid Measurement
C
Protein Quantification
D
Carbohydrate Detection
Understanding the Answer
Let's break down why this is correct
Answer
The biuret reagent is used to determine the concentration of proteins in a solution. It works by reacting with peptide bonds in proteins, producing a violet‑purple complex that can be measured spectrophotometrically. The intensity of the color is directly proportional to the amount of protein present. For example, a 1 mg mL⁻¹ protein solution will give a stronger violet color than a 0. 1 mg mL⁻¹ solution, allowing you to calculate the protein concentration from a standard curve.
Detailed Explanation
Biuret reagent reacts with peptide bonds in proteins. Other options are incorrect because DNA does not contain peptide bonds, so Biuret cannot react with it; Lipids are made of fatty acids and glycerol, not peptide bonds.
Key Concepts
Protein Measurement
Analytical Chemistry Methods
Biochemical Assays
Topic
Folin Phenol Reagent
Difficulty
hard level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1Why is the Folin phenol reagent particularly important in protein measurement?
easyBiological-sciences
Practice
2
Question 2Why is the Folin phenol reagent preferred for protein measurement over other reagents?
mediumBiological-sciences
Practice
3
Question 3The effectiveness of protein measurement using the Folin phenol reagent is largely dependent on its interaction with __________, which can lead to inaccurate results if not properly managed.
hardBiological-sciences
Practice
4
Question 4Order the steps involved in utilizing the Folin phenol reagent for protein quantification, starting from the sample preparation to the final measurement.
mediumBiological-sciences
Practice
5
Question 5Which of the following scenarios best illustrates the appropriate application of the Folin phenol reagent in protein quantification?
mediumBiological-sciences
Practice
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