Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
The pH of the sample should be neutral to avoid interference with the reagent.
B
The reagent should be used only at room temperature for optimal results.
C
The reagent's color change is solely indicative of protein presence, regardless of other substances.
D
The Folin phenol reagent should be prepared fresh for each experiment to maintain its activity.
Understanding the Answer
Let's break down why this is correct
Answer
The most critical thing is to make sure nothing else in the samples can reduce the Folin‑Ciocalteu reagent, because any reducing agent (like sugars, vitamins, or antioxidants) will also produce a blue color and falsely raise the protein reading. Before measuring, the researcher should remove or account for these substances, for example by using a purification step or by running a blank that contains the same matrix without protein. The assay must then be performed under the same pH, temperature, and incubation time for every sample and standard, so that the color development is comparable. For instance, if a blood plasma sample contains a high level of glucose, the researcher would need to either remove the glucose or correct the reading with a glucose‑only control to avoid over‑estimating protein concentration.
Detailed Explanation
The Folin phenol reagent loses its ability to react over time. Other options are incorrect because Some think the sample must be neutral, but the reagent works over a range of pH values; The reagent can be used at temperatures other than room temperature.
Key Concepts
Protein Quantification
Folin Phenol Reagent
Analytical Chemistry
Topic
Folin Phenol Reagent
Difficulty
easy level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1In pharmaceutical applications, how does the preparation of Folin Phenol Reagent influence laboratory procedures during the analysis of phenolic compounds?
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Question 2Why is the Folin phenol reagent particularly important in protein measurement?
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3
Question 3Why is the Folin phenol reagent preferred for protein measurement over other reagents?
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4
Question 4The effectiveness of protein measurement using the Folin phenol reagent is largely dependent on its interaction with __________, which can lead to inaccurate results if not properly managed.
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5
Question 5Order the steps involved in utilizing the Folin phenol reagent for protein quantification, starting from the sample preparation to the final measurement.
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6
Question 6Which of the following scenarios best illustrates the appropriate application of the Folin phenol reagent in protein quantification?
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7
Question 7A researcher is attempting to determine the concentration of a protein in an unknown sample using the Folin-Ciocalteu method. They prepare a standard curve using known concentrations of a standard protein solution and measure the corresponding absorbance values. What is the most critical factor the researcher must ensure when generating the standard curve for accurate quantification of the unknown sample?
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8
Question 8How does protein denaturation impact the accuracy of protein quantification methods like the Folin phenol reagent?
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