📚 Learning Guide
CRISPR-Cas9 Technology
easy

What is the primary reason CRISPR-Cas9 technology is effective in gene editing?

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Choose the Best Answer

A

It uses a guide RNA to target specific DNA sequences.

B

It randomly inserts genetic material into any part of the genome.

C

It relies solely on chemical reactions to alter DNA.

D

It is derived from human immune responses.

Understanding the Answer

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Answer

CRISPR‑Cas9 works because the Cas9 protein is guided by a short RNA that matches a specific DNA sequence, so it can locate and cut exactly where you want. This programmable targeting lets the system find and cleave a precise spot in the genome. After the cut, the cell’s repair machinery can be used to delete, add, or replace the DNA at that spot. The ability to direct the cut to almost any sequence makes the technique both powerful and efficient. For instance, a researcher can design an RNA that matches a faulty gene in a cell line, cut it out, and then insert a healthy copy in its place.

Detailed Explanation

The guide RNA is a short RNA strand that matches the DNA sequence you want to edit. Other options are incorrect because Some think CRISPR randomly inserts DNA, but it actually uses the guide RNA to find a single spot; CRISPR is not just a chemical reaction; it needs the Cas9 protein, which is a molecular scissors.

Key Concepts

Gene editing
CRISPR-Cas9 mechanism
Bacterial immune systems
Topic

CRISPR-Cas9 Technology

Difficulty

easy level question

Cognitive Level

understand

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