📚 Learning Guide
CRISPR-Cas9 Technology
easy

Arrange the following steps in the correct order for how CRISPR-Cas9 technology functions in gene editing: A) Cas9 protein cuts the DNA, B) Guide RNA binds to the target DNA sequence, C) The cell's repair mechanisms are activated, D) The target DNA sequence is recognized.

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Learning Path
Learning Path

Question & Answer
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Choose the Best Answer

A

A→B→C→D

B

B→D→A→C

C

D→B→C→A

D

C→A→B→D

Understanding the Answer

Let's break down why this is correct

Answer

The guide RNA first binds to the target DNA sequence (B). The complex then recognizes the target DNA (D). Cas9, guided by the RNA, makes a precise cut in the DNA (A). Finally, the cell’s repair machinery is triggered to fix the break (C). This sequence lets the cell incorporate or delete a piece of DNA as desired.

Detailed Explanation

The guide RNA first finds the exact DNA spot. Other options are incorrect because This answer says Cas9 cuts before the guide RNA binds; This option puts recognition before the guide RNA binds.

Key Concepts

Gene Editing
CRISPR-Cas9 Mechanism
Bacterial Immune System
Topic

CRISPR-Cas9 Technology

Difficulty

easy level question

Cognitive Level

understand

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