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Question & Answer
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It ensures that the Cas9 protein only binds to the target DNA sequence by recognizing the PAM site, preventing off-target effects.
It allows Cas9 to edit any DNA sequence without the need for a guide RNA, increasing efficiency.
It relies solely on the Cas9 protein's ability to cut DNA, with no impact from the guide RNA.
It makes the Cas9 protein less effective, as it binds randomly to various DNA sequences.
Understanding the Answer
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The Cas9 protein looks for a short DNA tag called a PAM (protospacer adjacent motif). Other options are incorrect because The guide RNA tells Cas9 where to go; Cas9 needs the guide RNA to find the target.
Key Concepts
CRISPR-Cas9 Genome Editing
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Deep Dive: CRISPR-Cas9 Genome Editing
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Definition
CRISPR-Cas9 Genome Editing is a revolutionary method used in genetic engineering that enables precise modifications of DNA sequences in living organisms. This technology utilizes the Cas9 protein, which acts as molecular scissors to cut DNA at specific sites, guided by a sequence known as Protospacer or guide RNA. The significance of this method in business lies in its potential applications across various fields, including medicine, agriculture, and biotechnology, allowing companies to innovate and develop solutions for complex challenges.
Topic Definition
CRISPR-Cas9 Genome Editing is a revolutionary method used in genetic engineering that enables precise modifications of DNA sequences in living organisms. This technology utilizes the Cas9 protein, which acts as molecular scissors to cut DNA at specific sites, guided by a sequence known as Protospacer or guide RNA. The significance of this method in business lies in its potential applications across various fields, including medicine, agriculture, and biotechnology, allowing companies to innovate and develop solutions for complex challenges.
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