Learning Path
Question & Answer
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The guide RNA binds to the target DNA sequence → B) Cas9 protein creates a double-strand break in the DNA → C) The cell's repair mechanisms are activated → D) The desired genetic modification is introduced during repair
Cas9 protein creates a double-strand break in the DNA → D) The desired genetic modification is introduced during repair → A) The guide RNA binds to the target DNA sequence → C) The cell's repair mechanisms are activated
The desired genetic modification is introduced during repair → A) The guide RNA binds to the target DNA sequence → B) Cas9 protein creates a double-strand break in the DNA → D) The cell's repair mechanisms are activated
The cell's repair mechanisms are activated → B) Cas9 protein creates a double-strand break in the DNA → A) The guide RNA binds to the target DNA sequence → C) The desired genetic modification is introduced during repair
Understanding the Answer
Let's break down why this is correct
The guide RNA first finds and sticks to the DNA that matches its sequence. Other options are incorrect because This option says Cas9 cuts before the guide RNA finds the DNA; This option places the genetic change before the cell repairs the break.
Key Concepts
CRISPR-Cas9 Genome Editing
medium level question
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Deep Dive: CRISPR-Cas9 Genome Editing
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Definition
CRISPR-Cas9 Genome Editing is a revolutionary method used in genetic engineering that enables precise modifications of DNA sequences in living organisms. This technology utilizes the Cas9 protein, which acts as molecular scissors to cut DNA at specific sites, guided by a sequence known as Protospacer or guide RNA. The significance of this method in business lies in its potential applications across various fields, including medicine, agriculture, and biotechnology, allowing companies to innovate and develop solutions for complex challenges.
Topic Definition
CRISPR-Cas9 Genome Editing is a revolutionary method used in genetic engineering that enables precise modifications of DNA sequences in living organisms. This technology utilizes the Cas9 protein, which acts as molecular scissors to cut DNA at specific sites, guided by a sequence known as Protospacer or guide RNA. The significance of this method in business lies in its potential applications across various fields, including medicine, agriculture, and biotechnology, allowing companies to innovate and develop solutions for complex challenges.
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