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CRISPR-Cas9 Genome Editing
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Arrange the following steps in the CRISPR-Cas9 genome editing process in the correct order: A) The guide RNA binds to the target DNA sequence, B) Cas9 protein creates a double-strand break in the DNA, C) The cell's repair mechanisms are activated, D) The desired genetic modification is introduced during repair.

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A

The guide RNA binds to the target DNA sequence → B) Cas9 protein creates a double-strand break in the DNA → C) The cell's repair mechanisms are activated → D) The desired genetic modification is introduced during repair

B

Cas9 protein creates a double-strand break in the DNA → D) The desired genetic modification is introduced during repair → A) The guide RNA binds to the target DNA sequence → C) The cell's repair mechanisms are activated

C

The desired genetic modification is introduced during repair → A) The guide RNA binds to the target DNA sequence → B) Cas9 protein creates a double-strand break in the DNA → D) The cell's repair mechanisms are activated

D

The cell's repair mechanisms are activated → B) Cas9 protein creates a double-strand break in the DNA → A) The guide RNA binds to the target DNA sequence → C) The desired genetic modification is introduced during repair

Understanding the Answer

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Answer

The guide RNA first binds to the target DNA sequence, directing the Cas9 protein to the exact spot. Cas9 then creates a double‑strand break at that location. Once the break is made, the cell’s repair mechanisms are activated to fix the damage. During this repair, the desired genetic modification is introduced, allowing the new sequence to be incorporated. For example, a single‑nucleotide change can be inserted into a gene to correct a disease‑causing mutation.

Detailed Explanation

The guide RNA first finds and sticks to the DNA that matches its sequence. Other options are incorrect because This option says Cas9 cuts before the guide RNA finds the DNA; This option places the genetic change before the cell repairs the break.

Key Concepts

CRISPR-Cas9 Mechanism
DNA Repair Processes
Gene Editing Applications
Topic

CRISPR-Cas9 Genome Editing

Difficulty

medium level question

Cognitive Level

understand

Practice Similar Questions

Test your understanding with related questions

1
Question 1

Arrange the following steps in the correct order for how CRISPR-Cas9 technology functions in gene editing: A) Cas9 protein cuts the DNA, B) Guide RNA binds to the target DNA sequence, C) The cell's repair mechanisms are activated, D) The target DNA sequence is recognized.

easyBiological-sciences
Practice
2
Question 2

In CRISPR-Cas9 genome editing, the Cas9 protein acts as molecular scissors that cut DNA at specific sites, guided by a sequence known as __________.

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