Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
The Cas9 protein randomly alters DNA sequences throughout the genome.
B
The guide RNA directs the Cas9 to a specific DNA sequence, enabling precise cuts.
C
The PAM site is a non-specific region that allows multiple edits at once.
D
The bacteria naturally develop resistance without any genetic modifications.
Understanding the Answer
Let's break down why this is correct
Answer
CRISPR‑Cas9 cuts a specific spot in the bacterial DNA where the antibiotic‑resistance gene is to be inserted. The Cas9 enzyme, guided by a short RNA sequence, creates a double‑strand break exactly at that site. The cell then repairs the break using a supplied DNA template that carries the resistance gene, a process called homologous recombination. In this way the new gene is precisely copied into the genome, giving the bacteria the ability to survive the antibiotic. For example, a plasmid containing a chloramphenicol‑resistance cassette can be inserted into the *gyrA* gene of *E.
Detailed Explanation
The guide RNA is made to match a specific DNA sequence. Other options are incorrect because Some think Cas9 changes DNA everywhere; The PAM site is a short DNA motif that Cas9 needs to bind.
Key Concepts
CRISPR-Cas9 Mechanism
Gene Editing Applications
Antibiotic Resistance
Topic
CRISPR-Cas9 Genome Editing
Difficulty
hard level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1How does CRISPR-Cas9 technology specifically improve the efficiency of gene editing in organisms?
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Question 2What is the primary reason CRISPR-Cas9 technology is effective in gene editing?
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3
Question 3How does CRISPR-Cas9 specifically target and edit genes in an organism?
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4
Question 4A research team is investigating a new method to modify the genome of a crop plant to enhance drought resistance. They consider using CRISPR-Cas9 technology for this purpose. Which aspect of CRISPR-Cas9 should the researchers prioritize to ensure the precision of their gene editing?
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Question 5How does the CRISPR-Cas9 system achieve precise genome editing?
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Question 6In CRISPR-Cas9 genome editing, how does the specificity of the Cas9 protein influence the success of gene editing?
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