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Question & Answer1
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Explore TopicChoose the Best Answer
A
It forms complexes with proteins that can be quantified spectrophotometrically.
B
It increases the pH of protein solutions, making them more soluble.
C
It acts as a preservative for protein samples before analysis.
D
It changes the color of proteins without affecting their concentration.
Understanding the Answer
Let's break down why this is correct
Answer
The alkaline copper solution is used in protein analysis because it reacts with the amino groups of proteins to form a colored complex that can be measured. This reaction allows scientists to quantify protein concentration by comparing the color intensity to a standard curve. The alkaline environment keeps the copper ions soluble and the reaction fast, which gives a reliable, reproducible signal. For example, when a protein sample is mixed with the solution, it turns greenish‑blue, and the darker the color, the more protein is present. Thus, the solution provides a quick, accurate way to determine how much protein is in a sample.
Detailed Explanation
The alkaline copper solution reacts with proteins to create a colored complex. Other options are incorrect because Some think it only raises pH to make proteins soluble; Others believe it preserves samples.
Key Concepts
Protein Analysis
Spectrophotometry
Colorimetric Detection
Topic
Alkaline Copper Solution
Difficulty
easy level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1How does the alkaline copper solution contribute to the colorimetric detection of proteins in biological samples?
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2
Question 2A biochemist is tasked with measuring the protein concentration in a new biological sample using the alkaline copper solution method. Which principle underlying this method is most crucial for ensuring accurate measurement of protein concentration?
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Practice
3
Question 3Which of the following examples illustrates the correct application of alkaline copper solution in protein analysis?
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Practice
4
Question 4In protein analysis, the Alkaline Copper Solution is primarily used to form complexes with proteins, which can then be quantified through __________.
easyBiological-sciences
Practice
5
Question 5What is the primary effect of using an alkaline copper solution in protein analysis?
mediumBiological-sciences
Practice
6
Question 6Which of the following statements about Alkaline Copper Solution and its role in protein analysis are true? Select all that apply.
hardBiological-sciences
Practice
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