Which of the following statements about Alkaline Copper Solution and its role in protein analysis are true? Select all that apply.

Alkaline Copper Solution is a staining method used after protein separation on a gel; it works by forming a complex between copper ions and the protein backbone in a basic environment, producing a visible brown band. The reaction is highly sensitive, detecting nanogram amounts of protein, and it does not require the harsh chemicals of traditional silver staining. Because the copper–protein complex is stable, the gel can be stored for a short time before the bands are visible. The solution is inexpensive, easy to prepare, and is often chosen when a quick, low‑cost protein detection is needed.