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Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
It increases the solubility of proteins in water
B
It facilitates the formation of colored complexes with proteins
C
It denatures proteins, making them easier to measure
D
It reduces the absorption of light by proteins in spectrophotometry
Understanding the Answer
Let's break down why this is correct
Answer
The alkaline copper solution is used to stain proteins that have been separated by electrophoresis, turning them into dark blue‑black bands on the gel. It works by binding copper ions to the amino groups in the protein, forming a colored complex that is easily visible. Because the copper complex is highly pigmented, faint proteins become detectable, improving the sensitivity of the analysis. For example, after running a gel, dipping it in the solution turns the protein bands dark, allowing you to see and quantify them. This staining step is the primary effect of using the alkaline copper solution in protein analysis.
Detailed Explanation
The copper ions in the alkaline solution bind to protein molecules and create a colored complex. Other options are incorrect because Students often think the solution makes proteins dissolve better; The main goal is not to break the protein structure.
Key Concepts
Protein analysis
Colorimetric detection
Spectrophotometry
Topic
Alkaline Copper Solution
Difficulty
medium level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1What are some of the primary applications of alkaline copper solutions in various industries, and what future prospects do they hold for business development?
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2
Question 2What is the correct sequence of steps for measuring protein concentration using an alkaline copper solution in a colorimetric assay?
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3
Question 3How does the alkaline copper solution contribute to the colorimetric detection of proteins in biological samples?
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4
Question 4Alkaline Copper Solution : Protein Detection :: Benedict's Reagent : ?
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5
Question 5Which of the following examples illustrates the correct application of alkaline copper solution in protein analysis?
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6
Question 6Why is the alkaline copper solution important in protein analysis?
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7
Question 7In protein analysis, the Alkaline Copper Solution is primarily used to form complexes with proteins, which can then be quantified through __________.
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8
Question 8Which of the following statements about Alkaline Copper Solution and its role in protein analysis are true? Select all that apply.
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