Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
It reacts with amino acids to produce a light-absorbing complex.
B
It denatures proteins, making them easier to measure.
C
It forms complexes with proteins that can be quantified spectrophotometrically.
D
It neutralizes acids in the sample to stabilize protein structures.
Understanding the Answer
Let's break down why this is correct
Answer
The alkaline copper solution reacts with proteins in a sample by reducing copper(II) ions to copper(I) in the presence of the protein’s amino‑acid residues, especially those containing phenylalanine or histidine. This reduction creates a blue copper‑protein complex that is stable in the alkaline environment. The intensity of the blue color is directly proportional to the amount of protein present, allowing the sample’s protein concentration to be measured by comparing the color to a standard curve. For example, when a serum sample is mixed with the solution, the resulting blue color can be read at 562 nm to determine the albumin level. This simple, color‑based reaction makes the assay quick, inexpensive, and easy to perform in most labs.
Detailed Explanation
The solution contains copper ions in a basic (alkaline) environment. Other options are incorrect because The misconception is that the solution reacts only with individual amino acids; The idea that it denatures proteins to make them easier to measure is incorrect.
Key Concepts
Protein Analysis
Spectrophotometry
Colorimetric Detection
Topic
Alkaline Copper Solution
Difficulty
hard level question
Cognitive Level
understand
Practice Similar Questions
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Question 1If the protein concentration in a sample is underestimated when using colorimetric assays, what is the most likely underlying cause of this effect?
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Question 2What is the correct sequence of steps for measuring protein concentration using an alkaline copper solution in a colorimetric assay?
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Question 3A biochemist is tasked with measuring the protein concentration in a new biological sample using the alkaline copper solution method. Which principle underlying this method is most crucial for ensuring accurate measurement of protein concentration?
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Question 4Alkaline Copper Solution : Protein Detection :: Benedict's Reagent : ?
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Question 5Which of the following examples illustrates the correct application of alkaline copper solution in protein analysis?
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Question 6Why is the alkaline copper solution important in protein analysis?
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7
Question 7In protein analysis, the Alkaline Copper Solution is primarily used to form complexes with proteins, which can then be quantified through __________.
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Question 8What is the primary effect of using an alkaline copper solution in protein analysis?
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Question 9Which of the following statements about Alkaline Copper Solution and its role in protein analysis are true? Select all that apply.
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