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Alkaline Copper Solution
easy

A biochemist is tasked with measuring the protein concentration in a new biological sample using the alkaline copper solution method. Which principle underlying this method is most crucial for ensuring accurate measurement of protein concentration?

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A

The alkaline copper solution reacts with proteins to form a stable color complex that can be quantified.

B

The alkaline copper solution denatures proteins, making them easier to measure.

C

The color produced by the alkaline copper solution is directly proportional to the volume of the protein sample.

D

The alkaline copper solution works best with only certain types of proteins based on their size.

Understanding the Answer

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The alkaline copper solution reacts with proteins to form a stable color complex. Other options are incorrect because Denaturing proteins does not help the measurement; The color intensity depends on protein concentration, not the sample volume.

Key Concepts

Protein Quantification
Colorimetric Assays
Spectrophotometry
Topic

Alkaline Copper Solution

Difficulty

easy level question

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Definition
Definition

Alkaline copper solution plays a critical role in protein analysis methods, facilitating the colorimetric detection of proteins in biological samples. This solution interacts with protein samples to form complexes that can be quantified using spectrophotometry, contributing to the accurate measurement of protein concentrations.

Topic Definition

Alkaline copper solution plays a critical role in protein analysis methods, facilitating the colorimetric detection of proteins in biological samples. This solution interacts with protein samples to form complexes that can be quantified using spectrophotometry, contributing to the accurate measurement of protein concentrations.

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