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Question & Answer1
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Explore TopicChoose the Best Answer
A
It provides a unique sequence that can be used to differentiate between closely related bacterial species.
B
It is primarily used for protein synthesis in eukaryotic cells.
C
It analyzes the morphology of bacterial cells to classify them.
D
It allows for the quantification of bacterial populations in a sample.
Understanding the Answer
Let's break down why this is correct
Answer
16S ribosomal RNA gene sequencing is useful because the 16S rRNA gene is present in all bacteria but contains both highly conserved regions that allow universal primers and variable regions that differ between species, so it acts like a DNA fingerprint. By amplifying and sequencing this gene, scientists can compare the variable segments to reference databases to identify which species a sample belongs to. The method is fast, inexpensive, and works even when the bacteria cannot be grown in the lab. For example, sequencing the 16S rRNA of a sample from a patient’s throat can reveal whether it contains *Streptococcus pneumoniae* or another related species, guiding appropriate treatment. Thus, 16S sequencing provides a reliable genetic marker for bacterial identification.
Detailed Explanation
16S rRNA gene sequencing looks at a part of the bacterial genome that is present in all bacteria but has small differences between species. Other options are incorrect because Some people think 16S rRNA is used for protein synthesis in eukaryotic cells; Another misconception is that 16S rRNA sequencing looks at bacterial shape or size.
Key Concepts
genetic markers.
Topic
16S Ribosomal RNA Gene Sequencing
Difficulty
easy level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1In the context of 16S ribosomal RNA gene sequencing, which approach allows for the identification of microbial taxa without the need for culture methods?
mediumBiological-sciences
Practice
2
Question 2Which of the following best describes the significance of 16S ribosomal RNA gene sequencing in understanding microbial communities through culture-independent methods?
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3
Question 3How has 16S ribosomal RNA gene sequencing revolutionized bacterial identification in clinical diagnostics, particularly through the use of genetic markers?
hardBiological-sciences
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4
Question 4A research team is conducting a study on bacterial diversity in the human gut. They decide to use 16S ribosomal RNA gene sequencing to identify the bacterial species present. Which of the following statements best explains why this method is advantageous for classifying these bacteria?
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Practice
5
Question 5In the context of classifying bacterial species through genomic approaches, 16S ribosomal RNA gene sequencing specifically targets the ________ gene, which is known for its high degree of conservation across different bacterial taxa.
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6
Question 6Arrange the steps for using 16S ribosomal RNA gene sequencing to classify bacterial species in a microbial community.
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7
Question 716S rRNA gene sequencing : bacterial classification :: DNA sequencing : ?
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8
Question 8What underlying mechanism explains why 16S ribosomal RNA gene sequencing can effectively classify diverse bacterial species in a complex microbial population?
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9
Question 9Which of the following statements about 16S ribosomal RNA gene sequencing are correct? (Select all that apply)
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Practice
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