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16S Ribosomal RNA Gene Sequencing
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Which of the following best describes the significance of 16S ribosomal RNA gene sequencing in understanding microbial communities through culture-independent methods?

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A

It allows for the identification of microbial species without the need for growth in a culture.

B

It requires the cultivation of bacteria in a lab setting to obtain accurate results.

C

It is exclusively used for determining antibiotic resistance in cultured bacteria.

D

It provides a method to analyze viral DNA sequences.

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16S ribosomal RNA gene sequencing reads a specific part of bacterial DNA that is present in all bacteria but varies slightly between species. Other options are incorrect because B suggests that growth of bacteria is necessary for sequencing; C states that the technique is only for antibiotic resistance.

Key Concepts

sequencing methods
culture-independent methods
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16S Ribosomal RNA Gene Sequencing

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medium level question

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Definition
Definition

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

Topic Definition

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

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