📚 Learning Guide
16S Ribosomal RNA Gene Sequencing
hard

In the context of classifying bacterial species through genomic approaches, 16S ribosomal RNA gene sequencing specifically targets the ________ gene, which is known for its high degree of conservation across different bacterial taxa.

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Learning Path

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Choose the Best Answer

A

18S rRNA

B

23S rRNA

C

16S rRNA

D

5S rRNA

Understanding the Answer

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Answer

In bacterial classification, 16S ribosomal RNA gene sequencing focuses on the 16S rRNA gene, a component of the small subunit of the ribosome. This gene is highly conserved, meaning its sequence changes very slowly over evolutionary time, which makes it useful for comparing different bacteria. Because of this conservation, the same gene can be amplified and sequenced across many species, allowing researchers to see how closely related they are. For example, sequencing the 16S rRNA gene of a soil bacterium and comparing it to a database can reveal whether it belongs to the same genus as a known pathogen. Thus, the 16S rRNA gene is the target used in genomic approaches to identify and classify bacterial species.

Detailed Explanation

The 16S rRNA gene is part of the ribosome, the cell’s protein‑making machine. Other options are incorrect because 18S rRNA is used to study eukaryotes, such as plants and animals, not bacteria; 23S rRNA is another ribosomal RNA, but it is larger and less conserved than 16S.

Key Concepts

16S ribosomal RNA gene sequencing
Bacterial classification
Genomic approaches in microbiology
Topic

16S Ribosomal RNA Gene Sequencing

Difficulty

hard level question

Cognitive Level

understand

Practice Similar Questions

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1
Question 1

Which of the following statements best explains the significance of 16S ribosomal RNA gene sequencing in identifying bacterial species as genetic markers?

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Question 2

In the context of 16S ribosomal RNA gene sequencing, which approach allows for the identification of microbial taxa without the need for culture methods?

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Which of the following best describes the significance of 16S ribosomal RNA gene sequencing in understanding microbial communities through culture-independent methods?

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How has 16S ribosomal RNA gene sequencing revolutionized bacterial identification in clinical diagnostics, particularly through the use of genetic markers?

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How does 16S Ribosomal RNA gene sequencing contribute to metagenomics and taxonomy through culture-independent methods?

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Question 6

A research team is conducting a study on bacterial diversity in the human gut. They decide to use 16S ribosomal RNA gene sequencing to identify the bacterial species present. Which of the following statements best explains why this method is advantageous for classifying these bacteria?

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Question 7

Arrange the steps for using 16S ribosomal RNA gene sequencing to classify bacterial species in a microbial community.

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Question 8

16S rRNA gene sequencing : bacterial classification :: DNA sequencing : ?

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Question 9

What underlying mechanism explains why 16S ribosomal RNA gene sequencing can effectively classify diverse bacterial species in a complex microbial population?

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