Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
18S rRNA
B
23S rRNA
C
16S rRNA
D
5S rRNA
Understanding the Answer
Let's break down why this is correct
Answer
In bacterial classification, 16S ribosomal RNA gene sequencing focuses on the 16S rRNA gene, a component of the small subunit of the ribosome. This gene is highly conserved, meaning its sequence changes very slowly over evolutionary time, which makes it useful for comparing different bacteria. Because of this conservation, the same gene can be amplified and sequenced across many species, allowing researchers to see how closely related they are. For example, sequencing the 16S rRNA gene of a soil bacterium and comparing it to a database can reveal whether it belongs to the same genus as a known pathogen. Thus, the 16S rRNA gene is the target used in genomic approaches to identify and classify bacterial species.
Detailed Explanation
The 16S rRNA gene is part of the ribosome, the cell’s protein‑making machine. Other options are incorrect because 18S rRNA is used to study eukaryotes, such as plants and animals, not bacteria; 23S rRNA is another ribosomal RNA, but it is larger and less conserved than 16S.
Key Concepts
16S ribosomal RNA gene sequencing
Bacterial classification
Genomic approaches in microbiology
Topic
16S Ribosomal RNA Gene Sequencing
Difficulty
hard level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1Which of the following statements best explains the significance of 16S ribosomal RNA gene sequencing in identifying bacterial species as genetic markers?
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Question 2In the context of 16S ribosomal RNA gene sequencing, which approach allows for the identification of microbial taxa without the need for culture methods?
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Question 3Which of the following best describes the significance of 16S ribosomal RNA gene sequencing in understanding microbial communities through culture-independent methods?
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4
Question 4How has 16S ribosomal RNA gene sequencing revolutionized bacterial identification in clinical diagnostics, particularly through the use of genetic markers?
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Question 5How does 16S Ribosomal RNA gene sequencing contribute to metagenomics and taxonomy through culture-independent methods?
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6
Question 6A research team is conducting a study on bacterial diversity in the human gut. They decide to use 16S ribosomal RNA gene sequencing to identify the bacterial species present. Which of the following statements best explains why this method is advantageous for classifying these bacteria?
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7
Question 7Arrange the steps for using 16S ribosomal RNA gene sequencing to classify bacterial species in a microbial community.
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8
Question 816S rRNA gene sequencing : bacterial classification :: DNA sequencing : ?
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9
Question 9What underlying mechanism explains why 16S ribosomal RNA gene sequencing can effectively classify diverse bacterial species in a complex microbial population?
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