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16S Ribosomal RNA Gene Sequencing
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How does 16S Ribosomal RNA gene sequencing contribute to metagenomics and taxonomy through culture-independent methods?

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A

It allows for the identification of microbial communities without the need for culturing organisms.

B

It solely focuses on the metabolic pathways of individual microorganisms.

C

It requires the cultivation of bacteria in laboratory settings to obtain accurate results.

D

It limits the analysis to only pathogenic species present in a sample.

Understanding the Answer

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The 16S rRNA gene is found in every bacterium and has parts that stay the same and parts that change. Other options are incorrect because Some think 16S sequencing tells us about the chemicals a microbe makes; A common mistake is to believe that bacteria must be grown first.

Key Concepts

metagenomics
taxonomy
culture-independent methods
Topic

16S Ribosomal RNA Gene Sequencing

Difficulty

hard level question

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understand

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Definition
Definition

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

Topic Definition

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

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