Learning Path
Question & Answer1
Understand Question2
Review Options3
Learn Explanation4
Explore TopicChoose the Best Answer
A
Sample collection → DNA extraction → PCR amplification → Sequencing → Data analysis
B
Sequencing → Sample collection → Data analysis → PCR amplification → DNA extraction
C
DNA extraction → Sample collection → Data analysis → Sequencing → PCR amplification
D
PCR amplification → Data analysis → Sequencing → Sample collection → DNA extraction
Understanding the Answer
Let's break down why this is correct
Answer
First, collect the microbial sample and extract total DNA, then amplify the 16S rRNA gene with universal primers by PCR. Next, sequence the amplicons using high‑throughput sequencing and clean the reads by trimming adapters and filtering low‑quality bases. Then cluster the cleaned reads into operational taxonomic units (OTUs) or amplicon sequence variants (ASVs) and compare each sequence to a reference database to assign taxonomic identities. Finally, compile the taxonomic assignments to build a profile of bacterial species present in the community. For example, a stool sample is processed this way to reveal the relative abundance of *Bacteroides* and *Lactobacillus* species.
Detailed Explanation
First you collect the sample, which gives you the bacteria you want to study. Other options are incorrect because This option starts with sequencing, but you cannot read DNA before you have it; It puts DNA extraction after sample collection, which is fine, but then it says data analysis before sequencing.
Key Concepts
16S ribosomal RNA gene sequencing
Microbial community profiling
Taxonomic classification of bacteria
Topic
16S Ribosomal RNA Gene Sequencing
Difficulty
easy level question
Cognitive Level
understand
Practice Similar Questions
Test your understanding with related questions
1
Question 1Which of the following statements best explains the significance of 16S ribosomal RNA gene sequencing in identifying bacterial species as genetic markers?
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Question 2In the context of 16S ribosomal RNA gene sequencing, which approach allows for the identification of microbial taxa without the need for culture methods?
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Question 3Which of the following best describes the significance of 16S ribosomal RNA gene sequencing in understanding microbial communities through culture-independent methods?
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Question 4How has 16S ribosomal RNA gene sequencing revolutionized bacterial identification in clinical diagnostics, particularly through the use of genetic markers?
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Question 5A research team is conducting a study on bacterial diversity in the human gut. They decide to use 16S ribosomal RNA gene sequencing to identify the bacterial species present. Which of the following statements best explains why this method is advantageous for classifying these bacteria?
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Question 6What is the primary purpose of 16S ribosomal RNA gene sequencing in microbiome studies?
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7
Question 7In the context of classifying bacterial species through genomic approaches, 16S ribosomal RNA gene sequencing specifically targets the ________ gene, which is known for its high degree of conservation across different bacterial taxa.
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Question 816S rRNA gene sequencing : bacterial classification :: DNA sequencing : ?
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Question 9What underlying mechanism explains why 16S ribosomal RNA gene sequencing can effectively classify diverse bacterial species in a complex microbial population?
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