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16S Ribosomal RNA Gene Sequencing
easy

Arrange the steps for using 16S ribosomal RNA gene sequencing to classify bacterial species in a microbial community.

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Learning Path

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Choose AnswerChoose the Best Answer

A

Sample collection → DNA extraction → PCR amplification → Sequencing → Data analysis

B

Sequencing → Sample collection → Data analysis → PCR amplification → DNA extraction

C

DNA extraction → Sample collection → Data analysis → Sequencing → PCR amplification

D

PCR amplification → Data analysis → Sequencing → Sample collection → DNA extraction

Understanding the Answer

Let's break down why this is correct

First you collect the sample, which gives you the bacteria you want to study. Other options are incorrect because This option starts with sequencing, but you cannot read DNA before you have it; It puts DNA extraction after sample collection, which is fine, but then it says data analysis before sequencing.

Key Concepts

16S ribosomal RNA gene sequencing
Microbial community profiling
Taxonomic classification of bacteria
Topic

16S Ribosomal RNA Gene Sequencing

Difficulty

easy level question

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understand

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Definition
Definition

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

Topic Definition

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

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