Practice Questions
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Which of the following statements best explains the significance of 16S ribosomal RNA gene sequencing in identifying bacterial species as genetic markers?
16S rRNA gene sequencing looks at a part of the bacterial genome that is present in all bacteria but has small differences between species. Other opti...
In the context of 16S ribosomal RNA gene sequencing, which approach allows for the identification of microbial taxa without the need for culture methods?
Culture-independent methods read the 16S rRNA gene directly from the sample. Other options are incorrect because Traditional culturing techniques grow...
Which of the following best describes the significance of 16S ribosomal RNA gene sequencing in understanding microbial communities through culture-independent methods?
16S ribosomal RNA gene sequencing reads a specific part of bacterial DNA that is present in all bacteria but varies slightly between species. Other op...
How has 16S ribosomal RNA gene sequencing revolutionized bacterial identification in clinical diagnostics, particularly through the use of genetic markers?
Sequencing reads the 16S rRNA gene, which is present in all bacteria but has unique regions for each species. Other options are incorrect because Sequ...
How does 16S Ribosomal RNA gene sequencing contribute to metagenomics and taxonomy through culture-independent methods?
The 16S rRNA gene is found in every bacterium and has parts that stay the same and parts that change. Other options are incorrect because Some think 1...
A research team is conducting a study on bacterial diversity in the human gut. They decide to use 16S ribosomal RNA gene sequencing to identify the bacterial species present. Which of the following statements best explains why this method is advantageous for classifying these bacteria?
The 16S rRNA gene contains parts that stay almost the same in all bacteria, called conserved regions. Other options are incorrect because The 16S rRNA...
What is the primary purpose of 16S ribosomal RNA gene sequencing in microbiome studies?
16S rRNA sequencing reads a part of the bacterial genome that is very similar across species but has small differences. Other options are incorrect be...
In the context of classifying bacterial species through genomic approaches, 16S ribosomal RNA gene sequencing specifically targets the ________ gene, which is known for its high degree of conservation across different bacterial taxa.
The 16S rRNA gene is part of the ribosome, the cell’s protein‑making machine. Other options are incorrect because 18S rRNA is used to study eukaryotes...
Arrange the steps for using 16S ribosomal RNA gene sequencing to classify bacterial species in a microbial community.
First you collect the sample, which gives you the bacteria you want to study. Other options are incorrect because This option starts with sequencing, ...
How does 16S rRNA gene sequencing contribute to understanding the human microbiome?
16S rRNA is a gene found in all bacteria. Other options are incorrect because People think sequencing shows what microbes do; The 16S rRNA gene is onl...
16S rRNA gene sequencing : bacterial classification :: DNA sequencing : ?
DNA sequencing reads the entire human genome. Other options are incorrect because Sequencing does not make proteins; Sequencing is not limited to viru...
A research team is studying a patient's gut microbiome to understand the impact of diet on microbial diversity. They decide to use 16S ribosomal RNA gene sequencing to analyze the sample. Which of the following statements best describes how this technique will aid their research?
16S rRNA gene sequencing reads a small, highly conserved part of the bacterial genome that acts like a barcode. Other options are incorrect because Th...
What underlying mechanism explains why 16S ribosomal RNA gene sequencing can effectively classify diverse bacterial species in a complex microbial population?
The 16S rRNA gene is almost the same in every bacterium, so it acts like a common language. Other options are incorrect because Some parts of the 16S ...
Which of the following statements about 16S ribosomal RNA gene sequencing are correct? (Select all that apply)
The 16S rRNA gene is a part of the bacterial ribosome that stays almost the same in all bacteria. Other options are incorrect because Some think 16S s...
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