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Folin Phenol Reagent
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A researcher is conducting an experiment to quantify protein concentration in a series of biological samples. They decide to use the Folin phenol reagent due to its sensitivity and simplicity. What is the most critical consideration the researcher should keep in mind when using this reagent to ensure accurate results?

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A

The pH of the sample should be neutral to avoid interference with the reagent.

B

The reagent should be used only at room temperature for optimal results.

C

The reagent's color change is solely indicative of protein presence, regardless of other substances.

D

The Folin phenol reagent should be prepared fresh for each experiment to maintain its activity.

Understanding the Answer

Let's break down why this is correct

The Folin phenol reagent loses its ability to react over time. Other options are incorrect because Some think the sample must be neutral, but the reagent works over a range of pH values; The reagent can be used at temperatures other than room temperature.

Key Concepts

Protein Quantification
Folin Phenol Reagent
Analytical Chemistry
Topic

Folin Phenol Reagent

Difficulty

easy level question

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Definition
Definition

The Folin phenol reagent is a key component in protein measurement methods, known for its sensitivity and simplicity. This reagent is utilized in various analytical procedures to detect proteins in different biological samples. Understanding the composition, stability, and reactions of the Folin phenol reagent is essential for accurate protein quantification.

Topic Definition

The Folin phenol reagent is a key component in protein measurement methods, known for its sensitivity and simplicity. This reagent is utilized in various analytical procedures to detect proteins in different biological samples. Understanding the composition, stability, and reactions of the Folin phenol reagent is essential for accurate protein quantification.

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