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16S Ribosomal RNA Gene Sequencing
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A research team is studying a patient's gut microbiome to understand the impact of diet on microbial diversity. They decide to use 16S ribosomal RNA gene sequencing to analyze the sample. Which of the following statements best describes how this technique will aid their research?

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A

It will provide a detailed analysis of all genes present in the bacteria, allowing for a comprehensive functional study.

B

It will identify and classify the bacterial species present in the sample, highlighting changes in diversity related to dietary factors.

C

It will isolate and culture each bacterial species found in the gut, enabling in-depth laboratory studies on their functions.

D

It will determine the metabolic activity of the gut bacteria, allowing researchers to understand their functional roles.

Understanding the Answer

Let's break down why this is correct

16S rRNA gene sequencing reads a small, highly conserved part of the bacterial genome that acts like a barcode. Other options are incorrect because The misconception is that sequencing shows every gene in the bacteria; The misconception is that the method isolates and grows each bacterium.

Key Concepts

16S ribosomal RNA gene sequencing
Microbial diversity
Gut microbiome
Topic

16S Ribosomal RNA Gene Sequencing

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medium level question

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Definition
Definition

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

Topic Definition

16S ribosomal RNA (rRNA) gene sequencing is a genomic approach used to classify bacteria based on the highly conserved 16S rRNA gene. This technique allows for the profiling and taxonomic classification of bacterial species present in complex microbial populations.

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